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KMID : 0380020040190060457
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 6 p.457 ~ p.461
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Production of Mycelium and Exopolysaccharides by Fed-batch Culture of Agaricus blazei
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Kim Hyun-Han
Na Jung-Geol
Chang Yong-Keun
Lee Sang-Jong
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Abstract
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DO-stat fed-batch cultures of Agaricus blazei were carried out using various feeding solutions, for the production of mycelial biomass and exopolysaccharides (EPS). It was observed to be more effective to use a feeding solution containing both carbon and nitrogen sources than that containing only carbon source. The best result was obtained when a feeding solution containing 450 g/l glucose, 60 g/l yeast extract, 30 g/l soytone peptone was used. The maxium mycelial biomass and EPS concentrations were 36.5 g/l and 10.9 g/l, respectively, at 100 hours of cultivation. The mycelial and EPS productivities were 0.37 g/l-h and 0.11 g/l-h, respectively. As compared with the batch culture, the mycelial biomass concentration and its productivity were 6.0- and 2.2-folds increased, respectively. The EPS concentration and its productivity were increased by 4.7 times and 1.8 times, respectively.
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KEYWORD
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Agaricus blazei, Fed-batch culture, Exopolysaccharides production
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